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Pig Immunoglobulin G(IgG) ELISA Kit

  • 中文名称:
    猪免疫球蛋白G(IgG)酶联免疫试剂盒
  • 货号:
    CSB-E06804p
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

产品详情

  • 产品描述:
        IgG是一种多功能的免疫球蛋白,具有与抗原结合、介导细胞毒性、激活补体等作用。在人体内,IgG可以通过调节免疫反应来清除感染和异常细胞,因此在疾病的诊断和治疗中有重要作用。
        华美生物所提供的Pig Immunoglobulin G(IgG) ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测猪血清、血浆、细胞培养上清、组织匀浆样本中的IgG,其灵敏度为0.467 μg/mL,检测范围为0.586 μg/mL-150 μg/mL。
  • 别名:
    N/A
  • 缩写:
    IgG
  • 种属:
    Sus scrofa (Pig)
  • 样本类型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围:
    0.586 μg/mL-150 μg/mL
  • 灵敏度:
    0.467 μg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IgG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:200 Average % 90  
    Range % 85-96  
    1:400 Average % 95  
    Range % 91-107  
    1:800 Average % 93  
    Range % 86-99  
    1:1600 Average % 91  
    Range % 88-97  
  • 回收率:
    The recovery of pig IgG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 88-98  
    EDTA plasma (n=4) 94 89-100  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    150 0.254 0.251 0.253    
    37.5 0.555 0.517 0.536    
    9.38 0.755 0.721 0.738    
    2.34 0.999 0.981 0.990    
    0.58 1.169 1.113 1.141    
    0 1.481 1.465 1.473    
  • 数据处理:
  • 货期:
    3-5 working days

引用文献

产品评价

平均分:
5.0分 - 1 个评价

样品类型:血清

样品信息:其他 猪

稀释比:1:100

产品评价: 标曲准确,样本数值在标曲范围之内,符合预期结果。

By 谢老师

靶点详情

  • 最新研究进展:
    IgG是一种免疫球蛋白,主要存在于血液和淋巴组织中。近年来的研究发现,IgG可以通过特异性结合抗原来识别和中和病原体,从而在机体的免疫防御中起着关键作用。除了作为一种免疫分子,IgG还被广泛用于治疗免疫相关疾病。最近的研究表明,IgG具有对自身免疫性疾病、恶性肿瘤和传染性疾病等多种疾病的治疗潜力。此外,研究人员还在探索IgG的新应用,例如在制备疫苗和免疫治疗中的应用等。