标签内参抗体
标签抗体
标签抗体，是指能够高度特异性识别通过重组技术构建的融合蛋白上特殊表位的抗体，可用于检测和纯化多种具有相同标记的蛋白质。抗原表位，又称抗原决定簇，是抗原分子中决定抗原特异性的特殊区域或基团，是与抗体特异性结合的结构或序列。随着生物技术的发展，科研人员可以通过DNA重组技术，构建包含目的基因以及表位标记的融合蛋白，进而通过特异性标签抗体对其鉴定与纯化，以达到研究的需求。
华美生物提供6*His、GFP、c-MYC、GST、E-Tag、HA-Tag、Flag Tag、Sumo Tag等标签抗体，通过抗原亲和纯化，可与相应的标记蛋白特异性结合，从而鉴定和纯化目标蛋白。种类齐全、特异性高，可保证您实验的顺利进行。
| Target | Applications | Target | Applications |
|---|---|---|---|
| 6*His | ELISA, WB | GFP | ELISA,WB,IP |
| c-MYC | ELISA, WB, IF, IP | GST | ELISA,WB |
| E-Tag | ELISA, WB, IP | HA-Tag | ELISA, WB, IF, FC, IP |
| Flag Tag | ELISA, WB, IP | Sumo tag | ELISA,WB |
内参抗体
内参即是内部参照（Internal Control），对于哺乳动物细胞表达来说一般是指由管家基因编码表达的蛋白(Housekeeping Proteins)，它们在各组织和细胞中的表达相对恒定，在检测蛋白的表达水平变化时常用它来做参照物。
华美生物提供的内参抗体包括Beta-actin、GAPDH、PCNA、TUBAIA等，性能稳定，质量可靠，可长期稳定储存，可在进行Western blot实验时检测目的产物、校正上样误差，也可作为空白对照，检测蛋白转膜是否完全、整个显色或者发光体系是否正常。
| Target | Applications | Target | Applications |
|---|---|---|---|
| GAPDH | ELISA, WB, IHC, IP, IF, FC | TUBA1A | ELISA, WB, IHC, IF, FC, IP |
| Beta-Actin | ELISA | PCNA | ELISA |
产品优势
- 种类齐全，特异性高
- 高灵敏度，可检测较低表达水平蛋白
Western Blot analysis of HA-Tag Monoclonal Antibody (CSB-MA000141M0m)
Western Blot analysis of E-Tag Monoclonal Antibody (CSB-MA000151M0m)
Western Blot analysis of GAPDH Monoclonal Antibody (CSB-MA000071M0m)
- 高稳定性，批内差/批间差较小
- 经过WB、FC、IP、IF、IHC等多重验证
HA-Tag Monoclonal Antibody (CSB-MA000141M0m)
Western Blot (WB)
Positive WB detected in: 3 different overexpression lysates with HA tagged
All lanes: HA-Tag antibody at 1:1000
Flow cytometry (FC)
Overlay histogram showing 293F transfected cells stained with CSB-MA000141M0m (red line) at 1:100.
Immunoprecipitation (IP)
Immunoprecipitating HA-Tag in 293F transfected whole cell lysate.
Lane 1: Mouse control IgG (1µg); Lane 2: CSB-MA000141M0m (5µg) + 293F transfected whole cell lysate (500µg); Lane 3: 293F transfected whole cell lysate (20µg)
Immunofluorescence (IF)
Immunofluorescence staining of 293F transfected cells with CSB-MA000141M0m at 1:100, counter-stained with DAPI.
GAPDH Monoclonal Antibody (CSB-MA000071M0m)
Western Blot (WB)
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate
All lanes: GAPDH antibody at 1:2000
Immunoprecipitation (IP)
Immunoprecipitating GAPDH in Hela whole cell lysate. Lane 1: Mouse control IgG; Lane 2: CSB-MA000071M0m (3μl) + Hela whole cell lysate (500μg); Lane 3: Hela whole cell lysate (20μg)
Flow cytometry (FC)
Overlay histogram showing Hela cells stained with CSB-MA000141M0m (red line) at 1:200.
Immunofluorescence (IF)
Immunofluorescence staining of Hela cells with CSB-MA000071M0m at 1:220, counter-stained with DAPI.
Immunohistochemistry (IHC)
IHC image of CSB-MA000071M0m diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system.
产品目录
| 产品名称 | 货号 | 规格 |
|---|---|---|
| HA-Tag Monoclonal Antibody | CSB-MA000141M0m | 100ul/50ul |
| TUBA1A Monoclonal Antibody | CSB-MA754656A0m | 100ul/50ul |
| E-Tag Monoclonal Antibody | CSB-MA000151M0m | 100μl/50μl |
| GAPDH Monoclonal Antibody | CSB-MA000071M1m | 100μl/50μl |
| GAPDH Monoclonal Antibody | CSB-MA000071M2m | 100μl/50μl |
| 6*His Monoclonal Antibody | CSB-MA000011M0m | 100μl/50μl |
| Myc tag Monoclonal Antibody | CSB-MA000041M0m | 100μg/50μg |
| Sumo tag Monoclonal Antibody | CSB-MA000131M0m | 100μg/50μg |
| MBP Monoclonal Antibody | CSB-MA000061M0m | 100μg/50μg |
| GAPDH Monoclonal Antibody | CSB-MA000071M0m | 100μl/50μl |
| GST Monoclonal Antibody | CSB-MA000031M0m | 100μg/50μg |
| Sumo tag Monoclonal Antibody | CSB-MA000132M0m | 100μg/50μg |
| GFP Monoclonal Antibody | CSB-MA000051M0m | 100μg/50μg |
| Flag Tag Monoclonal Antibody | CSB-MA000021M0m | 100μl/50μl |
| PCNA Monoclonal Antibody | CSB-MA000081M0m | 100μg/50μg |
| Beta-Actin Monoclonal Antibody | CSB-MA000091M0m | 100μg/50μg |
| Flag-Tag Monoclonal Antibody | CSB-MA000156 | 100μg/50μg |
| Myc-Tag Monoclonal Antibody | CSB-MA000157 | 100μg/50μg |
| HA-Tag Monoclonal Antibody | CSB-MA000158 | 100μg/50μg |
| His-Tag Monoclonal Antibody | CSB-MA000159 | 100μg/50μg |
引用文献
Myc tag Monoclonal Antibody referenced in "Borealin-nucleosome interaction secures chromosome association of the chromosomal passenger complex". The Journal of Cell Biology, 2019
His-Tag Monoclonal Antibody referenced in "When spider and snake get along: Fusion of a snake disintegrin with a spider phospholipase D to explore their synergistic effects on a tumor cell". Toxicon, 2019
Flag Tag Monoclonal Antibody referenced in "The role of the cytoplasmic tail of the influenza virus M2 protein in viral morphology and replication". University of Kent, 2018
His-Tag Monoclonal Antibody referenced in "Subfunctionalization of the Ruby2-Ruby1 gene cluster during the domestication of citrus". Nature Plants, 2018
