Mouse Angiotensin 1-7(Ang1-7)ELISA Kit

Mouse Angiotensin 1-7(Ang1-7)ELISA Kit

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中文名称：

小鼠血管紧张素1-7（Ang1-7）酶联免疫试剂盒
货号：

CSB-E13763m
规格：

96T/48T
价格：

￥3600/￥2500
其他：

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产品描述：

This Mouse Ang1-7 ELISA Kit was designed for the quantitative measurement of Mouse Ang1-7 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.
缩写：

Ang1-7
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

6.25 pg/mL-400 pg/mL
灵敏度：

1.56 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	93
	Range %	87-99
1:4	Average %	95
	Range %	89-102
1:8	Average %	95
	Range %	87-98

回收率：

The recovery of mouse Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-98
EDTA plasma (n=4)	95	91-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.745	2.721	2.733	2.597
200	2.316	2.245	2.281	2.145
100	1.923	1.898	1.911	1.775
50	1.467	1.366	1.417	1.281
25	0.796	0.788	0.792	0.656
12.5	0.458	0.447	0.453	0.317
6.25	0.258	0.242	0.250	0.114
0	0.138	0.134	0.136

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Potential therapeutic implications of calcitriol administration and weight reduction on CD4 T cell dysregulation and renin angiotensin system-associated acute lung injury in septic obese mice CL Yeh,Biomedicine & pharmacotherapy,2023
ApoE4 associated with severe COVID-19 outcomes via downregulation of ACE2 and imbalanced RAS pathway F Chen,Journal of translational medicine,2023
Recombinant human angiotensin-converting enzyme 2 plays a protective role in mice with sepsis-induced cardiac dysfunction through multiple signaling pathways dependent on converting angiotensin II to angiotensin 1–7 C Wu,Annals of Translational Medicine,2023
Renal angiotensin I-converting enzyme-deficient mice are protected against aristolochic acid nephropathy A Juretzko,Pflugers Archiv,2022
Imatinib and methazolamide ameliorate COVID-19-induced metabolic complications via elevating ACE2 enzymatic activity and inhibiting viral entry Zilun L,Cell Metabolism,2022
ACE2-like carboxypeptidase B38-CAP protects from SARS-CoV-2-induced lung injury Tomokazu Yamaguchi,nature communications,2021
Ambient fine particulate matter induced the elevation of blood pressure through ACE2/Ang(1–7) pathway: The evidence from urine metabolites X Du,Ecotoxicology and Environmental Safety,2020
B38-CAP is a bacteria-derived ACE2-like enzyme that suppresses hypertension and cardiac dysfunction T Minato,Nature Communications,2020
Ginsenoside Rg3 Attenuates Angiotensin II-Mediated Renal Injury in Rats and Mice by Upregulating Angiotensin-Converting Enzyme 2 in the Renal Tissue Liu H, et al,Evidence-Based Complementary and Alternative Medicine,2019
Effects of exendin-4 on the intrarenal renin-angiotensin system and interstitial fibrosis in unilateral ureteral obstruction mice: Exendin-4 and unilateral ureteral obstruction Ying Le.et al,SAGE Journals,2016
The effects of different angiotensin II type 1 receptor blockers on the regulation of the ACE-AngII-AT1 and ACE2-Ang(1-7)-Mas axes in pressure overload-induced cardiac remodeling in male mice. Wang X.et al,J Mol Cell Cardiol.,2016
Control of Adipogenesis by the Autocrine Interplays between Angiotensin (1-7)/Mas receptor and Angiotensin II/AT1 receptor Signaling Pathways Than A et al,J Biol Chem,2013

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.